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An innovator of life science tools

PCR / NGS Product Series

An innovation system of six technology platforms – from molecular enzyme engineering and molecular IVD to NGS library preparation, mRNA and monoclonal antibodies.

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Cleanroom and fermentation facilities (photos)
Insert image hereCleanroom and fermentation facilities (photos)images/pcr-ngs/pcr-ngs-hero.jpg
6
Technology platforms
20,000+
Clients & individual labs
5,000+ m²
mRNAtools plant area
10
Products with completed DMF filing

Series Overview

Three sections – choose one to open its details

Company & Customers

  • Company location
  • Customers: 20,000+ clients & individual labs
2 sections →

Technology Platforms

  • Directed evolution & rational design of molecular enzymes
  • High-density fermentation & ultra-clean purification
  • Molecular IVD platform
  • NGS library preparation platform
  • mRNA preparation platform
  • Monoclonal antibody platform
6 sections →

Manufacturing & Quality

  • UCF.ME ultra-clean molecular enzyme factory
  • mRNAtools manufacture factory
  • DMF filing
3 sections →

Highlights

An innovator of life science tools – from enzyme engineering to GMP-grade manufacturing

Six technology platforms

An innovation system covering the underlying technology and applied technology, from molecular enzymes to mRNA and antibodies.

Rational design & directed evolution

ZymeEditor™ rapidly improves fidelity, activity, sensitivity, specificity and thermostability of molecular enzymes.

3D-Optimizing & freeze-drying

Selected enzymes, optimized buffers and pre-designed primers/probes for PCR / LAMP; more than 97% of the water removed in freeze-drying.

NGS library preparation

Enzymatic-digestion DNA libraries, ultra-fast RNA libraries and a co-library method; compatible with MGI and Illumina sequencers.

Ultra-clean enzymes

DNA/RNA-free, endotoxin-free, sterile enzymes produced under the ISO 13485 quality management system.

20,000+ clients

Used by university laboratories, hospitals, NGS service providers, IVD kit companies, pharmaceutical companies and CRO/CDMO.

Company & Customers

Where our R&D centers, factories and warehouses are located, and who we serve.

Company LocationCustomers

Company Location

Company

Research and development centers (RDC), manufacturing bases and warehouses in China, Singapore and the USA. RDC = Research & Development Center.

Shanghai, China
  • Enzyme Evolution RDC
  • Protein / Antibody RDC
  • IVD RDC
  • Nucleic acid RDC
  • NGS RDC
  • 2 manufacture bases
Wuhan, China
  • Ultra Clean Factory for Molecular Enzyme
  • mRNAtools manufacture base
  • Biolink Internet Center
Singapore
  • SG Warehouse
USA
  • Maryland Business and Warehousing Center
  • Overseas R&D Center (under preparation)

Customers

Company

20,000+ clients & individual labs across research, clinical, diagnostic and pharmaceutical settings.

Customer types: university laboratories, hospitals and research institutes, NGS service providers, IVD test kit companies, clinical laboratories, pharmaceutical companies, CRO/CDMO companies
Insert image hereCustomer types: university laboratories, hospitals and research institutes, NGS service providers, IVD test kit companies, clinical laboratories, pharmaceutical companies, CRO/CDMO companiesimages/pcr-ngs/customer-types.jpg
Selected customer logos
Insert image hereSelected customer logosimages/pcr-ngs/customer-logos.jpg

Technology Platforms

Six technical platforms and the innovation system behind them – from molecular enzymes and IVD to NGS, mRNA and antibodies.

OverviewZymeEditor™Molecular DiagnosticNGSmRNAHieff Trans™

Technology Platform

Overview

We have built six technical platforms, and built an innovation system covering the underlying technology and applied technology.

Directed Evolution and Rational Design of Molecular Enzymes Platform

  • Rational design of molecular enzymes
  • Directed evolution of molecular enzymes based on an ultra-high-throughput single-cell sorting system

High Density Fermentation & Ultra-clean Protein Purification Platform

  • Molecular enzyme high-density fermentation technology
  • Ultra-clean molecular enzyme production technology (DNA-free, DNase/RNase-free)

Molecular IVD Platform

  • 3D-Optimizing of molecular diagnostic reagents
  • Freeze-drying technology for diagnostic preparations

NGS Library Preparation Platform

  • Enzymatic digestion method for NGS library preparation
  • Ultra-fast RNA library construction technology

mRNA Preparation Platform

  • mRNA stock solution preparation and analysis technology
  • mRNA LNP preparation and evaluation technology

High-quality Monoclonal Antibody Platform

  • Hybridoma monoclonal antibody preparation technology
  • Phage display technology
  • Single B cell antibody preparation technology

Rational Design and Directed Evolution

ZymeEditor™

ZymeEditor™ Protein Directed Evolution and Rational Design Platform

Rational design and directed evolution workflow toward an engineered enzyme
Insert image hereRational design and directed evolution workflow toward an engineered enzymeimages/pcr-ngs/zymeeditor-directed-evolution.jpg
Highlights
  • Rapid improvement of the key performance of molecular enzymes, including fidelity, activity, sensitivity, specificity, thermostability and substrate binding capacity, to meet application needs.
  • Rapid enrichment of beneficial mutation sites, shortening the evolutionary cycle, increasing the success rate of screening and significantly reducing costs.
Automated high-throughput single-cell sorting and isolation
Automated high-throughput single-cell sorting and isolation

Molecular Diagnostic Platform

Platform

3D-Optimizing of molecular diagnostic reagents and freeze-drying technology for diagnostic preparations.

Freeze-drying beads (photo)
Insert image hereFreeze-drying beads (photo)images/pcr-ngs/freeze-drying-beads.jpg
Advantages
  • 3D-Optimizing: to provide our customers with selected enzymes, an optimized reaction buffer and pre-designed primers/probes in compliance with PCR / LAMP technology.
  • Freeze-drying: removes more than 97% of the water, with a high success rate of freeze-drying.
  • Comprehensive applications: in vitro diagnosis, animal disease, early tumor screening, food safety detection, etc.
Water removed> 97%

NGS Platform

Platform

Library preparation technologies for DNA and RNA, supporting applications from basic research to clinical testing.

NGS platform overview: application fields, library preparation technology, library prep raw materials and basic research
Insert image hereNGS platform overview: application fields, library preparation technology, library prep raw materials and basic researchimages/pcr-ngs/ngs-platform.jpg
Highlights
  • Fast enzymatic digestion method for DNA library preparation
  • Ultra-fast RNA library preparation
  • Co-library technology that can distinguish between DNA and RNA sources
Advantages
  • Short time consumption, high efficiency, high fidelity and high stability
  • Applications in tumor diagnosis, genetic disease screening and microbial infection detection (mNGS)
  • Supports bioinformatic analysis and data mining
  • Compatible with MGI and Illumina sequencers

mRNA Preparation Platform

Platform

Post-transcriptional and co-transcriptional capping routes, from mRNA stock solution to LNP formulation and evaluation.

Post-transcriptional capping and co-transcriptional capping workflows for mRNA
Post-transcriptional capping (left) and co-transcriptional capping (right)
mRNA stock solution preparation and analysis technology
  • mRNA yield can reach 10 g/L, purity can reach more than 90%, and capping rate can reach more than 95%.
  • mRNA integrity detection, capping rate detection and Poly(A) tail length detection.
mRNA LNP preparation and evaluation technology

Mastering a series of detection techniques, from in vitro physical characterization and cell-level evaluation to animal-level evaluation.

Hieff Trans™ Advanced R&D Platform

Platform

AI-powered novel transfection reagent development platform – core R&D strengths powered by high-throughput compound library screening.

AI-Driven Molecular Design Platform
  • Utilizing AI-based molecular dynamics simulations and high-throughput virtual screening, the platform accurately predicts the structure–function relationships of transfection reagent molecules, rapidly identifying optimal modification sites.
  • By simulating interactions between transfection reagents and cell membranes, it optimizes the ratio of cationic density to hydrophobic groups, effectively reducing cytotoxicity while enhancing transfection efficiency.
High-Throughput Biological Validation Platform
  • Integrated with automated cell culture and flow cytometry systems, the platform enables high-throughput evaluation of transfection efficiency and cytotoxicity, accelerating the translation of candidate molecules from lab-scale discovery to industrial production.
  • Tailored to specific application scenarios, the platform has successfully screened optimal transfection reagents from over ten thousand candidate compounds and completed three successful pilot production batches.
Screening of modified molecules for Hieff Trans novel transfection reagents
Figure. Screening of modified molecules for Hieff Trans™ novel transfection reagents

Manufacturing & Quality

Ultra-clean enzyme production, a GMP-grade mRNA raw-material plant and completed DMF filings.

UCF.ME FactorymRNAtools FactoryDMF Filing

UCF.ME: Ultra Clean Molecular Enzyme Factory

Manufacturing

Ultra clean enzyme: the molecular enzymes are manufactured through high-density fermentation and an ultra-clean production process, ensuring the cleanliness (DNA/RNA-free, endotoxin-free, sterile, etc.) of enzymes under the ISO 13485 quality management system.

Fermentation tanks in the UCF.ME factory (photo)
Insert image hereFermentation tanks in the UCF.ME factory (photo)images/pcr-ngs/ucf-me-fermentation.jpg
Closed systemEnzymes are produced in a closed system with sterile tanks and tubing, to reduce potential environmental contamination.
Environmental controlThe factory is equipped with Class D, Class C, Class B and local Class A cleanrooms to prevent all kinds of contaminants.
Ultra-clean processA unique purification technology with more than ten procedures improves and guarantees the cleanliness and function of enzymes.
Large-scale productionIndustrial-grade facilities and chemical analysis equipment; five independent production lines with two 100 L, one 500 L and two 1,500 L fermentation facilities and matched purification systems.
Stable batchesControlled procedures ensure intra- and inter-batch consistency and promote high-standard, large-scale, stable preparation and long-term storage of molecular enzymes.

mRNAtools Manufacture Factory

Manufacturing

With an area of more than 5,000 square meters, the plant is equipped with automatic fermentation equipment and industrial-grade purification and freeze-drying facilities.

mRNAtools manufacturing plant: cleanroom corridor, fermentation and purification equipment (photos)
Insert image heremRNAtools manufacturing plant: cleanroom corridor, fermentation and purification equipment (photos)images/pcr-ngs/mrnatools-factory.jpg
GMP gradeThe plant is designed and constructed in accordance with GMP standards for pharmaceuticals, and in compliance with the ISO 13485:2016 quality management system.
Product packageRaw and auxiliary materials including enzymes, nucleotides and SAM are provided for downstream mRNA vaccine production and drug preparation, to meet the high standards for vaccine production and certification registration requirements.
High production capacityThe supply capacity of mRNA vaccine raw materials will be increased from 1 billion doses/year to 10 billion doses/year.

DMF Filing

Quality

Completed DMF filing of several products.

Filed products

DMF filing
S/NProduct nameApplication type / number
1T7 RNA PolymeraseMF037035
2UltraNuclease GMP-gradeMF037298
3mRNA Vaccinia Capping Enzyme GMP-gradeMF037433
4mRNA Cap 2′-O-Methyltransferase GMP-gradeMF037434
5Pyrophosphatase, Inorganic GMP-gradeMF037545
6Murine RNase inhibitor GMP-gradeMF037546
7BspQI GMP-gradeMF037607
8Deoxyribonuclease I (DNaseI) GMP-gradeMF037744
9Salt Active UltraNuclease GMP-gradeMF037815
10PEI Transfection ReagentMF038217

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Email: sales@yuanchuangbiotech.com  ·  Tel (Vietnam): 0352507835